Anti-ATP-citrate synthase/ACLY

Application

Research CategoryApoptosis & Cancer

Anti-ATP-citrate synthase/ACLY Antibody | ABC426 is an antibody against ATP-citrate synthase/ACLY Antibody for use in Western Blotting, Immunohistochemistry.

Research Sub CategoryApoptosis - Additional

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected ATP-citrate synthase/ACLY in human pancreas and human prostate tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

ATP citrate synthase, also known as ATP-citrate (pro-S-)-lyase (ACL) or Citrate cleavage enzyme, and encoded by the gene name ACLY is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis. In nervous tissue, ATP citrate-lyase may be involved in the biosynthesis of acetylcholine.

Immunogen

Epitope: Unknown

KLH-conjugated linear peptide corresponding to human ATP-citrate synthase/ACLY.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected ATP-citrate synthase/ACLY in 10 µg of A549 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~120 kDa observed